Control MAb shows slightly higher staining in the glands GL and lamina propria asterisk. The numbers of pooled tissue specimens for each RNA sample were as follows: For an unknown reason the mRNA levels in the stomach were comparable to several other segments of the gastrointestinal canal and distinctly lower than in the esophagus which repeatedly showed high mRNA signals and relatively weak or moderate immunohistochemical staining. Optimal dilutions should be determined by each laboratory for each application. Discussion SP protein has been recently considered a potential biomarker of cancer due to its frequent expression in different types of tumor tissues [ 18 , 19 ].
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CST – SP Antibody
Place tube back in magnetic separation rack. Vanon Goat IgG Control. SP calcium-binding protein overexpression is associated with immortalization of human breast epithelial cells in vitro and early stages of breast cancer development in vivo. In the tumor categories studied, the expression of SP showed no correlation to the tumor grade data not shown.
The supernatant is the cell lysate. Immunohistochemical differentiation of high-grade prostate carcinoma from urothelial carcinoma.
Anti-SP binding protein antibody (ab) | Abcam
Immunohistochemical results indicated that the placenta indeed expresses very high levels of SP protein in trophoblast cells Fig. Add to cart Quantity. The highest immunoreactions were localized in the epithelial cells of the superficial epithelial cuff region, while the reactions became weaker toward the crypts.
Pre-wash magnetic beads just prior to use:. In addition, positive intracellular reactions were observed, which most probably reflects either the newly produced protein in the cytosolic compartment of the cell or the fraction of SP molecules that interact with F-actin-binding protein ezrin [ 23 ].
SP caonn is a relatively small 95 amino acid isoform of the S protein family that was first isolated from human placenta [ 2 ]. S100l contributions SPar participated in the design of the study, tissue processing, immunohistochemical staining and light microscopy and drafted the first version of the manuscript. Scrape cells off canonn plate and transfer to microcentrifuge tubes. The second most prominent immunostaining was observed in the gastric mucosa.
This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1. Appropriate isotype controls are highly recommended in order to show specific binding in your primary antibody immunoprecipitation. Log in Remember me.
Datasheet References Protocols Overview.
SP protein has been recently considered a potential biomarker of cancer due to its frequent expression in different types of tumor tissues [ 1819 ]. Protein A Magnetic Beads: Collection of specimens was approved by the local ethics committees.
S100P Antibody #7677
Please refer to primary antibody datasheet or product webpage for recommended antibody dilution. SP is 95 aa in length and contains short, sequential modules. S proteins primarily mediate immune responses in various tissue types but are also involved in neuronal development The specificity of the MAb was also evaluated by immunohistochemistry in comparison with the control MAb in parallel sections of the human stomach antrum Fig.
Gastric carcinomas showed the strongest immunoreactions, almost half of the specimens having strong signals. It also varied within each tumor category, which is demonstrated in breast Fig.
Based on the high expression in certain tumors, SP could represent a potential target for novel diagnostic and therapeutic applications. Preparation and Storage Reconstitution. In breast cancer cell lines, SP overexpression seems to be an early event that has been suggested to play a role in the immortalization of human breast epithelial cells in vitro and tumor progression in vivo [ 3 ].
Moreover, its direct implication in cancer biology has been proposed on the basis of the experimental data obtained with S10p tumor cells in vitro and in vivo as well as of the data from various gene array studies [ 891520 – 22 ]. Published online Feb Learn how your comment data is processed.
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